International Journal of Biology Research

International Journal of Biology Research


International Journal of Biology Research
International Journal of Biology Research
Vol. 1, Issue 1 (2016)

Culturing and sensitivity of diabetic foot ulcer of the patients visiting to Ayub medical complex Abbottabad, Pakistan


Salma Afridi, Aneeqa Iqbal, Shakeel Ahmed, Syed Javed Shah, Muhammad Waqas, Muhibullah Afridi, Anhum Aslamn, Mujadad ur Rehman

Diabetic foot ulcer is one of the most serious complications occurring in diabetic patients causing amputation. This study was done to identify the different species of microorganisms in infection of patients visiting to Ayub Medical Complex. Gram positive and Gram negative bacteria were isolated such as Streptococcus, Staphylococcus, Escherichia coli and Klebsiella pneumonia by swab culturing method on different media such as blood agar and MacConkey agar media and sensitivity test were performed by disc diffusion method on nutrient agar for both Gram positive and Gram negative bacteria. In this study Gram positive is mostly present in diabetic foot ulcer patients. Staphylococcus was mostly present in patients i.e. 63 out of 100 patients have Staphylococcus in their infection. By performing sensitivity test for these bacteria Staphylococcus is mostly resistive to Ampicillin, Cefazolin, Oflaxacin, Streptomycin while mostly sensitive to Amikacin (53.96 %), Gentamycin (25.39 %), Cifroflaxin (34.92 %) and Vancomycin (15.87 %). Streptococcus was sensitive to Gentamycin (27.27 %), Linezolid (36.3 %), Pipracillin tozobactum (36.3 %), Vancomycin (27.2 %) and resistive to Cefazolin, Ampicillin and Doxycycline. Gram negative bacteria Klebsiella pneumonia was sensitive to Amikacin, Vancomycin, Lincomycin and mostly resistive to Doxycycline, Meropenem and Cefazoline. Escherichia coli was mostly sensitive to Erythromycin (25 %), Oflaxacin (50 %), Azactam (50 %), Amikacin (50 %) and resistive to Doxycycline, linomycin and Gentamycin. This study conclude that Amikacin, Vancomycin and Erythromycin are mostly sensitive against both Gram negative and Gram positive bacteria.
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