International Journal of Biology Research

International Journal of Biology Research


ISSN: 2455-6548

Vol. 1, Issue 1 (2016)

A Study on the Phytochemical, Proximate and Antimicrobial Properties of Root Extract of Nauclea latifolia Smith

Author(s): Chukwuma O Maureen, Akachukwu E Esther, Adimonyemma N Ruffina, Iroka F Chisom
Abstract: The phytochemical, proximate and antimicrobial properties of Nauclea latifolia were investigated using standard procedures. Fresh leaves were collected, washed, shredded, sundried before milling into powder and then subjected to qualitative phytochemical, proximate and antimicrobial analysis. The phytochemical screening recorded the presence of bioactive important substance such as tannins, flavonoids, saponins, steroids, phenols and cardiac glycoside in the leaves. The proximate analyses revealed that the dry leaves contain high amounts of ascorbic acid (66mg/100g), crude fiber (14.44%), carbohydrate (38.67%) and appreciable amounts of protein (20.05%). The powdered samples were extracted in five different solvents (hot and cold ethanol; hot and cold water and cold ether), and tested against five pathogenic organisms (Staphylococcus aureus, Candida albicans, Escheriachia coli, Klebsiella pneumonia, and Proteus sp.). The effects of the leaf extracts and a standard antibiotic (Ciprofloxacin) which served as the control were used to compare the potency of the leaf extracts. The leaf extracts recorded reasonable zones of inhibitions (5-12mm) suggesting susceptibilities of the micro-organisms to root extract in some of the solvents used.
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