International Journal of Biology Research

International Journal of Biology Research


ISSN: 2455-6548

Vol. 1, Issue 1 (2016)

Propagation of Rose (Rosa Hybrida L.) Under Tissue Culture Technique

Author(s): Faiza Nizamani, Ghualm Shah Nizamani, Muhammad Rashid Nizamani, Saeed Ahmed, Nazeer Ahmed
Abstract: The rose is the most popular ornamental plant in the world, as well as the most important cut flower. Throughout history no other plant has such wide appeal and been the center of so much attention than the Rose. Roses are one of the world's most important ornamentals for a long time and are most often used for ornamental, medicinal and aromatic purposes. The experiment was conducted at Tissue Culture Laboratory; Plant Breeding and Genetics Division, Tando Jam during 2014. The aim of present investigation was to determine appropriate basal medium and growth regulators for in vitro propagation of Rosa hybrida from nodal meristem explants. The basal medium of Murashige and Skoog (1962) containing with different concentrations of MS + 30 g L-1 sugar, MS + BAP 0.5 mg L-1 + 30 g L-1 sugar, MS + IBA 0.1 mg L-1 + BAP 5 mg L-1 + 30 g L-1 sugar, MS + NAA 0.5 mg L-1 + BAP 0.5 mg L-1 + 30 g L-1 sugar, MS + NAA 0.1 mg L-1 + BAP 2 mg L-1 + 30 g L-1 sugar for shoot induction and MS½ + 30 g L-1 sugar, MS½ + NAA 1 mg L-1 + 30 g L-1 sugar, MS½ + NAA 2 mg L-1 + 30 g L-1 sugar, MS½ + IBA 1 mg L-1 + 30 g L-1 sugar, MS½ + IBA 2 mg L-1 + 30 g L-1 sugar for root induction were used in this study. The statistical analysis of variance showed that days to initiation, number of shoots, shoot length, number of leaves, number of roots and root length were highly significant at 5% probability level. The results showed that early days to initiation was recorded, maximum number of shoots bottle-1, shoot length bottle-1 and number of leaves bottle-1 were recorded under the concentration of MS + NAA 0.1 mg L-1 + BAP 2 mg L-1 + 30 g L-1 sugar, followed by number of shoots bottle-1, shoot length bottle-1 and number of leaves bottle-1 were obtained under the concentration of MS + NAA 0.5 mg L-1 + BAP 0.5 mg L-1 + 30 g L-1 sugar and minimum number of shoots bottle-1, shoot length bottle-1 and number of leaves bottle-1 were recorded under the concentration of MS + 30 g L-1 sugar. The results indicated that maximum number of roots and root length were achieved under MS½ + IBA 2 mg L-1 + 30 g L-1 sugar and minimum number of roots and root length were recorded under MS½ + 30 g sugar L-1. IBA is an auxin plant growth regulator used to promote and accelerate root formation of plant. It was concluded from this study that MS + NAA 0.1 mg L-1 + BAP 2 mg L-1 + 30 g L-1 sugar for shoot induction and MS½ + IBA 2 mg L-1 + 30 g L-1 sugar proved best for root induction in rose.
Pages: 23-27  |  2631 Views  1835 Downloads
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