Soil Microorganisms as potential source of novel and or improved products of industrial importance have gained highest importance in Microbial technology and bioprocess engineering. The present study was carryout the isolated the multi-enzyme producing bacteria from agricultural field at Arni, Thiruvannamalai District, Tamilnadu, India. Totally 26 morphologically different colonies were isolated namely SCBT01 to SCBT26 and checked different enzyme activities such as Amylase, Protease, Lipase and Cellulase. There are 12 strains it ability to produce the enzymes and particularly SCBT06 it produced Amylase, Protease and SCBT19 it produced the Amylase, Protease, Lipase and Cellulase in this strain Concerted as potential strain. In the strain to suggest to industry which have the capacity to produce commercially important enzymes on industrial scale; Microbial enzymes are generally cheaper to be produced and are obtained in high yields.