Vol. 1, Issue 4 (2016)
Synthesis and evaluation of antimicrobial potential of copper nanoparticle against agriculturally important Phytopathogens
Author(s): SS Shende, ND Gaikwad, SD Bansod
Abstract: Biosynthesis of stable copper nanoparticles was achieved using Ocimum sanctum leaf extract. These biosynthesized copper nanoparticles were characterized by using UV-Vis spectroscopy showed absorption of copper nanoparticles at 345nm. FTIR spectrum of copper nanoparticles suggests that copper nanoparticles are surrounded by different organic molecules such as terpenoids, alcohols, ketones, esters, aldehydes and carboxylic acid. TEM analysis showed that the copper nanoparticles were rod, cylindrical and elliptical. The average particle size of copper nanoparticles was 25 nm with total concentration 300.29 particles/frame, 13.53 particles/ml and -14.9 mV zeta potential value. The maximum antibacterial activity of copper nanoparticles was found against Xanthomonas axonopodis pv. punicae (17.25±0.95) while Rhizoctonia solani (10±0.81) showed minimum activity with 0.01 mg/ml and 0.03mg/ml MIC respectively. The maximum antifungal activity of copper nanoparticles was found against Alternaria carthami (18.5±1.7) while the minimum activity was found against Rhizopus stolonifer (10.5±0.5). The MIC of the copper nanoparticles against fungus was found to be 0.06 mg/ml for Alternaria carthami, Aspergillus niger, Fusarium oxysporum f.sp udum and 0.03 mg/ml for Colletotrichum gloeosporioides, Colletotrichum lindemuthianum, and Drechslera sorghicola, Fusarium oxysporum f.sp. carthami, Fusarium oxysporum f.sp. ciceri, Macrophomina phaseolina, Rhizoctonia bataticola and Rhizopus stolonifer. Thus, copper nanoparticles can be exploited in the field of agriculture in formulation of various biopesticides, insecticides and ecologically feasible effective management strategy against harmful phytopathogens.