International Journal of Biology Research

International Journal of Biology Research


ISSN: 2455-6548

Vol. 3, Issue 1 (2018)

Preliminary phytochemical screening of bioactive compounds from leaves of Centella asiatica L. Urban

Author(s): Dr. Mariappan Senthilkumar
Abstract: The present study described that the preliminary phytochemical analysis of leaf extracts of Centella asiatica. The organic solvents such as hexane, chloroform, ethyl acetate and ethanol were used for extracting bioactive compounds present in leaf. The phytochemical tests of the different extracts have revealed the presence of different quantities of alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins, saponins and reducing sugars. Among all the extracts, ethanolic extract of leaf showed maximum yield of bioactive compounds than the other solvent extracts. Carbohydrates and falvonoides were present in all four extracts and hexane and chloroform extracts were yielded similar bioactive compounds Ethanol extracts showed the presence of all compounds. Proteins, antheroquinones and amino acids were absent in all the extracts. Thin layer chromatographic profiles yielded the different pattern of compounds and as well as different Rf values. The number of bands and Rf values of each extracts and in suitable solvent systems. Five different types of solvent systems were used to evaluate better elution of compounds. In specific compounds such as alkaloids detection from ethanol extract yielded three bands in solvent system of ethanol: NH4OH (8.5:1.5), saponins yielded five bands in chloroform: glacial acetic acid: ethanol: water (6:2:1:1), terpenoids yielded eight bands in benzene: ethyl acetate (5:5) and flavonoids yielded ten bands in chloroform: ethanol (9:1).
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