Vol. 3, Issue 1 (2018)
Evaluation of genetic diversity within the populations of the Indian false vampire bat, Megaderma lyra deduced by RAPD – PCR
Author(s): Deep Narayan Prasad, Ram Kumar, Dr. V Elangovan
Abstract: Megaderma lyra is a non-migratory carnivorous bat and maintains high level of roost fidelity and its dispersal ability is still obscure. The present work was conducted to examine genetic diversity within the populations of M. lyra. RAPD-PCR approach was used to assess the genetic diversity within five colonies located at Barabanki, Uttar Pradesh, India. The RAPD-PCR data illustrates quite a low level of diversity within five populations. A total of 59 bands were amplified with the 6 primers OPA-11, OPAA-09, OPW-02, OPB-18, OPR-08, and OPA-13 with an average of 9.8 bands per primer. The molecular weight ranged from 100-900 bp. The polymorphic loci within the populations of M. lyra varied over 46.5%. Among the primers used OPR-08 produced a maximum of 12 bands. The similarity coefficient values ranged from 0.432 to 1.00 while the polymorphic information content (PIC) ranged from 0.651 (OPAA-09) to 0.720 (OPA-11). The alleviated level of heterozygosity reveals that the population will be less resilient to threats and less adaptive.