Vol. 3, Issue 1 (2018)
Isolation and screening of multiple enzyme producing Alkaliphilic Bacillus species from Lonar Lake
Author(s): Lodha Chitra Kundanmal
Abstract: Lonar Lake is a unique ecosystem in India and was formed by meteorite impact on basaltic rock. It is situated in a village Lonar in the Buldhana district of Maharashtra State, India. Lonar Lake has been reported for the presence of diverse bacterial genera which are able to produce a number of different enzymes. These enzymes particularly lipases, proteases and amylases show their activity at high pH. The objective of this study was to isolate multienzyme producing Bacillus species from Lonar Lake source. In the present study, a total of 20 water samples were collected from Lonar Lake in sterilized bottle. 28 Bacillus species were isolated and identified on the basis of morphological and biochemical characteristics as well as screened for amylase, protease and lipase enzyme production. Among the 28 isolates, three isolates were found multienzyme producers namely AB03, AB10 and AB21. These isolates were confirmed by 16srRNA. Among these three isolates AB10 isolate was found most prominent species for multienzyme production. The promising single strain which can produce multienzyme has potential to be used in various economic industrial processes as it can withstand high pH conditions.