Vol. 4, Issue 1 (2019)
Identification of antibacterial potential of Phenolic compounds from Garcinia Mangostana fruit pericarp extracts against pathogenic bacterium in freshwater fish Cirrhinus mrigala
Author(s): Pandian Dhanalakshmi, Venkatachalam Ramasubramanian
Abstract: G. mangostana fruits are the preserved part of this plant and are essential for the pleasant flavor. So, G. mangostana fruit was named as the ‘queen of tropical fruits’ fruit pericarp extract has been demonstrated the antimicrobial activity against pathogenic organisms. The present study was conducted to evaluate the antibacterial activity of G. mangostana fruit pericarp in various solvents against P. fluorescens in freshwater fish C. mrigala. G. mangostana fruit pericarp powder (20g) was extracted in 450 mL of each solvent (petroleum ether, Toluene-99.5%, ethyl acetate-99%, methanol -99%). Antibacterial activity was investigated. Vancomycin (VA30/disc) was used as positive control. Minimum inhibitory concentration was tested by using serial dilution method. GC-MS (Gas chromatography mass spectrometry) spectral analysis was used for mass spectral identification was an Aux Detector [Aux 1 MS Transfer line (C) 280] to identify phytochemical compounds of G. mangostanafruit pericarp extract. The antibacterial maximum inhibitory activity was produced by methanol extract (17.3 ± 1.53) against P. fluorescens. While minimal activity was found from petroleum ether extract (3.3±1.53). Minimum inhibitory concentration (MIC) was observed in P. fluorescens was 10 mg of extract, 10µl of bacterial sample in the dilution factor of10-1 to 10-5.In this study, G. mangostana possess antibacterial activity against P. fluorescens. Further analysis is needed for the isolation and identification of active principles present in the extracts which could apparently be worked for fish feed formulation use and also the disease prevention technique through feed in the freshwater aquaculture system.