Vol. 4, Issue 3 (2019)
Evaluation of some tumors markers in rats induced with Diethyl nitrosamine and hepatoprotective role of some antioxidants
Author(s): Samir A Zaahkouk, Ahmed BM Mehany, Sawsan A El Shamy, Salah M EL Sharkawy
Abstract: Hepatocellular carcinoma is one of the most common malignancies worldwide and in Egypt the most common form of primary liver cancer. HCC represent the second most prevalent cancer among men in Egypt. HCC occurs in a number of some conditions that commonly includes hepatitis C and B, alcoholic, schistosomiasis, aflatoxins (AFB) and cirrhosis diethyl nitrosamine (DEN) is a well-known strong hepatocarcinogenic agent. It is known that DEN induces damage in enzymes involved in DNA repair and is normally used to induce liver cancer in experimental animal models as rats. Curcumin is a strong anti-inflammatory agent and anti-cancer effects with strong therapeutic potential against a variety of cancers. Vitamin C is an important free radical scavenger in extracellular fluids, trapping radicals and protecting bio membranes from peroxide damage and tissues damage of the liver. AFP was found to be a weak diagnostic predictor due to not specific and with low sensitivity. Objective: To evaluate the early detection of HCC by a novel biomarkers than AFP, and the hepatoprotective role of curcumin and vitamin C. Materials and methods: This study was conducted on a patch of 90 adult mature healthy male albino rats (Rattus rattus) averaged weight (190 ± 10 g) were allowed to acclimatize in the laboratory and distributed into 9 groups 10 rats for each. Serum lipid profile and some tumors biomarkers were measured for all groups at the end of experiment. Results: The results showed that the level serum total cholesterol, TG, LDL-C risk ratios revealed a significant increased for them and decreased HDL-C in DEN group when compared with its corresponding level in control group, and when treated with curcumin and vitamin c the results showed a significant decrease when compared with DEN group. Receiver Operator of Characteristics (ROC) curve analysis of AFP revealed that cut off value was ˃3.62 (ng/ml) leading to 79.7% for sensitivity, 76.0% specificity, 81.2% positive Predictive value, 80.1 negative Predictive val revealed that ue, and 78.4 for accuracy, while (ROC) curve analysis of Alpha L- Fucosidase revealed that cut off value was ˃285 (ng/ml) leading to 91.2% for sensitivity, 90% specificity, 90.9% positive Predictive value, 94.7 negative Predictive value, and 89.7 for accuracy and (ROC) curve analysis ofSCCAg1 had cut off value was ˃124.8 (ng/ml) leading to 95.5% for sensitivity, 81% specificity, 93.8% positive Predictive value, 90.1 negative Predictive value, and 92.9 for accuracy Conclusion: AFU and SCCA1 are more accurate marker than AFP for early detection or diagnosis of HCC. AFU and SCCA1 showed higher sensitivity, specificity and correlated more to other indices than AFP. Antioxidants like curcumin and vitamin c administration improved the lipid profile and the parameters of tumors biomarkers.