Commonly the reference values used in most of the laboratory are based on the population. These usually do not have similarity with the Indian population; this is seen especially in case of the lipid profile. Clinicians all over the world use these reference values to interpret the results obtained in patients, it should correctly impersonate the defined population having close comparison with the patient coming for investigation. There is no well documented evidence for the establishment of reference levels for lipids parameters for the Indian population, so there is a need for large scale study on lipid profile of Indian population. Materials and Methods: This is a cross-sectional study, including 200 subjects in total (according to CLSI guidelines). We analyzed total cholesterol, triglycerides, HDL-cholesterol by standard methods and, LDL-cholesterol was calculated using Friedewald’s formula. Results: Median values and ranges (2.5–97.5th percentile) in mg/dL for fasting and nonfasting total cholesterol (TC), triglycerides (TG), HDL-C, VLDL, LDL-C were: 116–239 (189), 38.8–172.4 (84), 30.9–72 (48), 7.7–42 (17.2), 59.5–177.0 (124.6), 119.9–255.1 (189), 43.9–278.6 (120), 25.5–67.1 (44), 8.7–55.7 (24.6), 50.8–178.6 (118.6), respectively. After grouping them according to age and sex, we observed that HDL-C was significantly wider in females compared to males, TC was not significantly wider, but TG was higher in males. In the age group 39–48 years TC, TG, HDL-C and LDL-C in males were higher as compared to females of that age group. Conclusion: Reference ranges are wider as compared to western standard, which is due to variation in diet, ethnicity, environment, etc. Hence this can be considered as the reference interval for this study population, further study is needed in a larger population.