Antimicrobial activity of bacteriocins produced by different strains of Bacillus Thurigiensis (BT) against some indictors bacteria, under different experimental conditions
Fahdah Alshammari, Anthony Ejiofor, Terrance Johnson
The project objective was to scrutinize the effect of bacteriocins fashioned by Bacillus Thuringiensis (Bt) strains have gotten from Middle Tennessee (USA) on three bacterial strains, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus, under different experimental conditions which were temperature, pH, and incubation time. The clear inhibition zone on plates (20mm, 12mm) indicated that 32 Bt strains out of 66 tested Bt strains had produced bacteriocin. It was apparent that no bacteriocin was produced at 15 hours of incubation, while a maximum production was noted at incubation time of 24 h. Our results had generally declared the effect of the pH and the temperature on the Bt antimicrobial activity through bactriocince production, that is, almost all bacteriocins’ strains of Bt exhibited antibacterial activity at pH between 7-9 against all bacteria used as indicators, and bacteriocins production was sensitive to higher temperatures.