International Journal of Biology Research

International Journal of Biology Research


International Journal of Biology Research
International Journal of Biology Research
Vol. 3, Issue 4 (2018)

Comparative Study on the in vivo antioxidant properties of Moringa oleifera and Camellia sinensis on MSG-induced oxidative-stressed rats


MB Ayoola, IE Ezeagu, NC Ejiofor

Introduction: The production of reactive oxygen species (ROS) is usually implicated in oxidative stress which is widely reported to be induced by monosodium glutamate (MSG), a known flavour enhancer commonly used as food seasoning. Oxidative stress is known to play key role in the development of various disease conditions. The in vivo antioxidant properties of both Moringa oleifera and Camellia sinensis were both investigated in MSG- induced oxidatively stressed rats. Methods: Moringa oleifera leaves were obtained from the local market while popular branded Camellia sinensis was purchased from a supermarket in Enugu, Nigeria. The dried plant materials were powdered using a clean electric blender. Hundred grams (100 g) of dried, ground sample of both samples were extracted using the maceration method. The extracts were evaporated to dryness using a rotary evaporator and stored at 4oC until use. Antioxidant properties of both plant samples were also determined. Twenty male wistar rats were divided into five groups of four rats each. The rats were administered with 0.6mg/kg body weight (b.w) dose of MSG solution for 14 days to induce oxidative stress, the control group was given distilled water. Subsequent treatment with MO, GT and combination of both extracts for a period of 28 days was carried out. One group was left untreated. The antioxidant levels and lipid profile of the rats were investigated. Results: The antioxidant properties of the sample extracts showed the presence of flavonoid (135.14 ± 5.20 and 208.24 ± 14.38), and phenolics (62.85 ± 1.70 and 91.68 ± 0.22) for both Moringa oleifera and Camellia sinensis respectively. The plant samples both showed in vivo and in vitro nitric oxide scavenging properties. The plant samples also presented an increased catalase enzyme activity and the lipid profile analyses showed no significant difference at 95% level of probability. Both plants reversed the histopathological damage that occurred in MSG-induced oxidative stressed rats. Conclusion: This study confirmed the usefulness of the medicinal plants: Camellia sinensis, Moringa oleifera and their combination in alleviating common medical conditions through the antioxidant properties.
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How to cite this article:
MB Ayoola, IE Ezeagu, NC Ejiofor. Comparative Study on the in vivo antioxidant properties of Moringa oleifera and Camellia sinensis on MSG-induced oxidative-stressed rats. International Journal of Biology Research, Volume 3, Issue 4, 2018, Pages 26-32
International Journal of Biology Research