A study of the extended-spectrum beta-lactamases and ambler class C beta-lactamases in environmental samples

Michael U Agama

A study of the extended-spectrum beta-lactamases and ambler class C beta-lactamases in environmental samples

Authors
Michael U Agama

Abstract
Extended-spectrum beta-lactamases (ESBLs) and Ambler class C lactamases (Amp C) have emerged to provide bacteria with formidable resistance to modern drugs. The current study aimed to examine the presence of ESBL and Amp C producing isolates among environmental samples in the rural communities of Enugu State, Nigeria. A total of 42 isolates were screened and observed for the presence of ESBL and Amp C producing isolates. The double-disc approximation method was used, the production of either of the two enzymes was detected by observing zones of inhibition in diameters. The result showed the presence of ESBL and Amp C in the samples tested. The work reveals that Escherichia coli (8) is the significant ESBL producer, followed by Pseudomonas aeruginosa and Klebsiella pneumoniae (6). The observed synergistic effect in the zone of inhibition between the disc of Ceftazidime and Amoxicillin-clavulanic acid indicates the presence of ESBL. In contrast, Amp C's presence was characterized by no observation of synergism in the zone of inhibition between the disc of Ceftazidime and Amoxicillin-clavulanic acid disc. This study recommends adequate and appropriate detection and monitoring of ESBL and Amp C producing enzymes in rural communities.

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