The experiment was carried out in Tissue Culture Laboratory, Plant Breeding and Genetics Division, Nuclear Institute of Agriculture Tando Jam. The Four wheat varieties viz. Amber, Khirman, Sarsabz and Soghat were used with different concentrations of phytohormones. The concentration of 2, 4-D, Picloram and NAA (2 mg l^-1) for callus induction, MS + indole-3-acetic acid (IAA), Kinetin (Kin) and indole-3-butyric acid (IBA) for regeneration of plantlets while, MS + IBA with different concentrations of sugar percent were used for root induction in wheat. The result showed that maximum proliferation and callus induction was achieved in Amber variety with 2, 4-D, maximum plant regeneration with concentration of MS + IAA 5.0 mg L^-1 + 4.0 mg L^-1 Kin+ 30 g sugar L^-1 and more number of roots were developed under the concentration of MS + IBA 2.0 mg L^-1 + 30 g sugar L^-1 were achieved for immature embryo culture in wheat.