International Journal of Biology Research

International Journal of Biology Research


International Journal of Biology Research
International Journal of Biology Research
Vol. 1, Issue 2 (2016)

Production of Cellulase by Aspergillus niger NCFT 4263.10 using Agro waste as a Substrate


Bijay Kumar Sethi, Asutosh Shyamaprasad Dash, Amrita Satapathy, Subodh Kumar Tripathy, Sidharth Parida, Bikash Chandra Behera

Aspergillus niger NCFT 4263.10 was evaluated for the production of cellulase by liquid static surface fermentation (LSSF), Liquid shaking fermentation (LShF) and solid state fermentation (SSF). Higher cellulase and protein secretion was observed when fermentation carried out in LSSF condition as compared to ShF and SSF. Maximum cellulase production was observed in LSSF (393.5±3.41U/ml) when wheat bran was used as the substrate followed by banana peels and saw dust. Immobilized spores of A. niger applied for fermentation up to five cycle and study revealed that when wheat bran was used as the carbon source, the activity of cellulase was found to be maximum. Highest Cellulase activity observed at second cycle (415.4±6.5U/ml) followed by third cycle (379.24 ± 2.65U/ml), fourth cycle (340.07± 2.6U/ml) and fifth cycle (204.25± 1.71U/ml)
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How to cite this article:
Bijay Kumar Sethi, Asutosh Shyamaprasad Dash, Amrita Satapathy, Subodh Kumar Tripathy, Sidharth Parida, Bikash Chandra Behera. Production of Cellulase by Aspergillus niger NCFT 4263.10 using Agro waste as a Substrate. International Journal of Biology Research, Volume 1, Issue 2, 2016, Pages 23-27
International Journal of Biology Research