International Journal of Biology Research

International Journal of Biology Research


International Journal of Biology Research
International Journal of Biology Research
Vol. 1, Issue 2 (2016)

Micropropagation of Jatropha maheshwarii Subr. & Nayar, an endemic species restricted to the southern terminus of India


Christal John Pushparathi BEN, Vaithialingam SIVANADANAM

An efficient micro propagation protocol for mass multiplication of Jatropha maheshwarii, a traditionally important endemic species is described. Explants were collected from its natural habitat to derive plantlets through In vitro technique. Morphogenetic potential of somatic explants like shoot tip, axillary bud and leaf discs were studied by culturing them on Murashige and Skoog’s medium, either alone or in combinations, with various plant growth regulators namely benzyl amino purine, indole-3-acetic acid, naphthalene acetic acid, kinetin and 2, 4-dichlorophenoxyaceticacid. Inoculated shoot tips expressed 90% shoot growth with BAP+IAA (1mg/l each) treatment. However, with axillary bud explants BAP in combination with KIN (1mg/l and 0.5mg/l) showed profuse shoot budding as well as multiple shoot formation in a faster pace at 95% response rate. Inoculated leaf disc explants showed callus proliferation all over the surface of explants on medium augmented with 2, 4-D (2.0 mg/l). Further caulogenesis and rhizogenesis were also noticed in about 45 days. The shoots rose from shoot tip and axillary bud explants were transferred to rooting medium containing NAA and IBA, to enhance root formation. Where, IBA (1mg/l) seems to be an effective hormone for rhizogenesis with 90% rooting response. The plantlets regenerated were hardened efficiently and transferred to the field successfully with 80% survival rate. This protocol can be used for the mass propagation of the species and thereby conserving its valuable genetic resource.
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How to cite this article:
Christal John Pushparathi BEN, Vaithialingam SIVANADANAM. Micropropagation of Jatropha maheshwarii Subr. & Nayar, an endemic species restricted to the southern terminus of India. International Journal of Biology Research, Volume 1, Issue 2, 2016, Pages 11-15
International Journal of Biology Research