Substances released by healthy and intact roots into the environment are collectively designated as root exudates. In present study, Salvadora persica Linn. Was used as an experimental plant as it has a number of proven medicinal applications and almost all parts of this plant have pharmacological importance. Root exudates were collected from S. persica and carbohydrate and amino acids were measured quantitatively. Protein/amino acids estimation were done by the methods Ninhydrin and Bradford’s colorimetric assay while carbohydrate estimation was done by the methods Anthrone and Phenol-sulfuric Acid colorimetric assay. In the rhizosphere, root exudate plays an important role as attracting signal molecules to attract the plant growth promoting microbes (endophytes and rhizobacteria) which performs important plant growth promoting functions (biocontrol, hormone production and bioremediation). Result of this study showed the presence of carbohydrate and amino acids in root exudates of S. persica, which will help plant in the promotion of plant growth, immunity and increase the resistance to arid and saline conditions which is not only of economic importance but also counted as contribution to the agriculture field.