Hypothyroidism is most common autoimmune disorders generally caused by an attack on the thyroid gland it results in inflammation and damage of the thyroid cells, linked to hormonal, human immune system under pathophysiology and psychological stress. The study focuses on the level of thyroid and stress hormone and its impact on the immune system, and oxidative stress. Two hundred human subjects were randomly selected. Hormonal analysis was conducted of both thyroid and stress hormones and level of cytokines, both pro and anti-inflammatory and oxidative stress was performed using serum samples. Statistical analysis was performed of all the parameters. The study depicts significant increase in TSH and decrease in T3 and T4 hormones. Also rise in stress hormones was observed. The rise in the hormones resulted in significant increase in the level of pro and decrease in anti-inflammatory cytokines. Increase in oxidative stress have lead to increase in normal process of the body, the production of free radicals, the reactive oxygen species (ROS), GSH, GPx, LPx which naturally created stress in the body. The level of superoxide dismutase (SOD), repaired cells and reduced the damage done by superoxide. It also acts as anti-inflammatory, neutralizing the free radicals maintaining the levels of hormones which proved to be same in hypothyroidism when compared with the normal groups. Apart from the physical and emotional point of view, our study focused on the immunological changes in the body in correlation with other metabolic processes in patients of hypothyroidism caused due to stress.