The study was conducted to assess the level of knowledge and practice of medical professionals in Shaheed Suhrawardy medical college hospital, Dhaka on hospital waste management. Total 105 respondents were interviewed. Mean age of the respondents was (38.10±10.857) years. About 72.4% was female and 89.5% of respondents were Muslim. Mean years of schooling of the respondents was (11.70±7.846) years and mean income of the respondents was (31914.29±25361.28) tk. About 36.19% was nurses, 28.57% of the respondents were doctors and 35.24% was cleaner. Mean professional experience of the respondents was (12.69±10.92) years. All the respondents had knowledge about hospital waste. About 99% of the respondents had knowledge about sharpe waste, pathological waste, liquid waste and general waste. About 99% respondents collected hospital waste in specific color container and 98.08% had knowledge about waste disposal. Near about 97.1% of the respondent’s practices of waste disposal was dumping and 99% of them practices incineration as waste disposal. Among them, 92.7% respondents told that the best procedure of waste disposal is dumping. About 98.1% of the respondents had very good knowledge and (94.2%) of them had very good practices. All of the respondents told it was essential to have knowledge about waste disposal. About 45.1% of the respondents told that the source of information about waste disposal was advocacy and seminar, seminar and training. We found satisfactory knowledge and practices of hospital waste management among the study population. If this level of knowledge and practices are available in every hospital of Bangladesh, both patients and medical professionals will be safe from unwanted infections.