In present investigation, we conducted a case control-based genetic screening of single nucleotide polymorphisms (SNPs) loci in TSHR gene with the aim to explore the relationship between the candidate SNPs and the susceptibility to hypothyroidism (HPO) in central India. A genetic polymorphism study of TSHR was undertaken using a polymerase chain reaction-restriction fragment length polymorphism method the patterns of genotype and allele distribution in both groups suggested a non-significant association between TSHR D727E gene polymorphism hypothyroidism in central India population. Body mass index was found to be significantly higher in female and male HPO patient group than in female and male healthy controls (P = 0.0001).