This study aimed the investigation of bla_CTX-M-15 gene into 73 enterobacteria collected by the Observatoire de la Résistance des Microorganismes aux Anti-Infecteux en Côte d’Ivoire (ORMICI) in Abidjan. The identification of the species was carried out by standard microbiological methods and confirmed by Maldi-Tof. The antibiotic susceptibility was performed by Vitek-2 and the phenotypic characterization of ESBLs was carried out by double synergy test. The presence of bla_CTX-M-15 genes was carried out by PCR followed by sequencing. 56.2% of the strains were ESBL producers. The ESBLs were resistant to cefotaxime (57.5%), ceftazidime (57.5%) and cefepime (54.8%). High resistance to gentamicin (60.3%), ciprofloxacin (60.3%), colistin (79.4%) and cotrimoxazol (60%) was also observed. Carbapenem were the most effective on these strains. 95.1% of ESBLs harbored the bla_CTX-M-15 gene. Our study confirms predominance of CTX-M-15 among Enterobacteriaceae throughout the world.