The present study was undertaken to study the Physico-chemical properties of water and species of Spirogyra in Tapti pond of Multai, District Betul (M.P.). The study revealed the presence of different species of Spirogyra. Spirogyra is cosmopolitan, freshwater, filamentous green algae, represented by about 300 species in world. Most of them are green floating in stagnant ponds and pools, a few species are attached and some occur in running water. Spirogyra is commonly known as pond silk, water silk, pond scum or mermaid’s trees because of their bright green silky appearance.