Structural and functional annotation of streptococcus pneumonia hypothetical protein – In silico Approach
S Sugunakala, T Akilandeswari, TR Barath Kumar, S Subasri, N Nivetha, R Akiladevi
The pathogenic and resistant nature of Streptococcus pneumonia causing pneumococcal infections can be comprehended by their structurally and functionally annotated sequences. However, many proteins of this organism are not annotated so far and are categorized as hypothetical proteins that means, the in - vivo functions for that proteins are yet to be built up. These hypothetical proteins may play a vital role in infectious process and be considered as a drug target. Identification of a good drug target is an important step in drug discovery process. The main aim of this work is to annotate the hypothetical protein in S. pneumonia using bioinformatics tools. Initially, the organism specificity and sub cellular localization were analyzed. Then, the domain analysis was carried out which showed the presence of glycosyl dehydrogenase domain in this hypothetical protein. Further, the functional annotation analysis was also carried out which showed that this hypothetical protein plays a role in human - pathogen interaction pathway namely glycan degradation pathway. Since there is no structural evidence for this protein, efforts were made to analyze and to predict the primary, secondary and tertiary structure. Then the predicted tertiary structure was validated and submitted to protein modeling database. Further, the phylogenetic analysis revealed that there was a significant sequential level of conservation among the query hypothetical protein sequence and the template structural sequences. Finally, active site analysis was carried out in which the amino acid residues and their positions were also identified. By knowing the nature of amino acid residues and area of the active sites novel inhibitors may be designed and used to inhibit the host - pathogen interaction occur through glycan degradation pathway and may results in reduced rate of occurrence of pneumococcal infections.
S Sugunakala, T Akilandeswari, TR Barath Kumar, S Subasri, N Nivetha, R Akiladevi. Structural and functional annotation of streptococcus pneumonia hypothetical protein – In silico Approach. International Journal of Biology Research, Volume 2, Issue 3, 2017, Pages 77-81