A pilot study of caries preventive effect of xylitol against plasmid born biofilm producing Lactobacillus acidophilus isolated from human dental caries
S Vijayalakshmi, S Rajasekar, A Mohankumar
Today, tooth decay usually happens slowly over a period of years. Decay begins when the bacterial population increases in the mouth. A cavity forms when acids accumulate and cause more damage to the tooth. The cariogenic infection is caused from predominant pathogen Lactobacillus acidophilus. So the plaque samples were collected from different childhood caries around varied dental clinics around Tirupur Dt. The organisms confirmed by phenotypic and genotypic characterization. After the organism was screened for biofilm production to check the resistant ability of cariogenic pathogen. Next the resistant was confirmed by plasmid analysis. In this study 10 biofilm producing cariogenic isolates (MTVG02, MTVG06, MTVG17, MTVG36, MTVG38, MTVG75, MTVG85, MTVG92, MTVG95 and MTVG120) having 10000 – 600bp. Due to this resistance ability of organism urgently need therapeutic drug to fight the predominant pathogen. Currently, xylitol has a powerful mechanical action to eliminate a cariogenic pathogen around oral cavity. Different concentration of xylitol (3%, 8%, 12% and 18%) was performed by Agar Well Diffusion assay. Among the concentration the maximum inhibitory activity was recorded 3% concentration of xylitol against Lactobacillus acidophilus.
S Vijayalakshmi, S Rajasekar, A Mohankumar. A pilot study of caries preventive effect of xylitol against plasmid born biofilm producing Lactobacillus acidophilus isolated from human dental caries. International Journal of Biology Research, Volume 2, Issue 4, 2017, Pages 17-22