Free radicals are the naturally formed molecules in the body which possess several roles. Under stress conditions, their production may increase which may cause deleterious effects. Free radicals are scavenged efficiently by plant products which are having various type of phytochemicals including phenolic compounds and flavonoids. The present study focused on the quantification of the total phenolic and flavonoid content in the methanolic extract of the Mesua ferrea and determination of its antioxidant potentials and antimicrobial activity. The study showed that plant extract was having a significant amount of phenolic and flavonoid content. The extract was efficiently scavenging the DPPH radicals and showed a considerable total antioxidant and ferric reducing power. For the antimicrobial study, gram-positive as well as gram-negative bacteria were used, and the extract was showing the ability to inhibit their growth. Thus, M. ferrea may be a potential candidate as a future antioxidant and antimicrobial agent.