Enteric fever is still an important public health problem in developing countries including India. A changing antibiotic susceptibility pattern of Salmonella serovars and emergence of multi drug resistance has increased to a great concern. Aim of the study was to investigate the antibiotic susceptibility pattern of Salmonella species. Study was carried out at the Department of Post-Graduate Studies & Research in Biological Science, R.D University, Jabalpur. A total of 40 clinical samples were collected from typhoid patient of different hospitals. Antibiotic susceptibility test was performed by Kirby-Bauer disc diffusion method and results were interpreted by National Committee for Clinical Laboratory (NCCLS) guideline. All the 16 isolates of Salmonella were tested against 14 antimicrobial compound namely cefotaxime, norfloxacin, aztreonam, ceftriaxone, nalidixic acid, nitrofurantoin, cefuroxime, gentamycin, amikacin, ciprofloxacin, ofloxacin, ceftazidime, cefixime and cefdinir. All the mentioned drugs are the common drug of choice for the treatment of typhoid fever. Seven isolates of Salmonella were found resistant to 14 of these antimicrobial compounds. However, 7 isolates were found resistant to cefotaxime, norfloxacin, aztreonam, ceftriaxone, nalidixic acid, nitrofurantoin, cefuroxime, gentamycin, amikacin, ciprofloxacin, ofloxacin, ceftazidime, cefixime and cefdinir. It was observed that Salmonella isolated from stools sample had developed simultaneous resistance to commonly used antibiotics. It was observed that Salmonella isolated from stool had developed simultaneous resistance to commonly used antibiotics. This has led to enormous morbidity in and around Jabalpur region. The area around Jabalpur is representative of tropics, where tropical diseases are abundantly present. The illiteracy, bad food habits temperature and water logging are main cause of food borne and water borne diseases, typhoid is much common.