The present study has been under taken to find out the antibacterial activity of methanol and water extract of Loligo duvauceli ink. The antibacterial activity was screened against E. coli and S.aureus. Different concentrations was prepared and tested the bacterial strains for their inhibitory activities using simple disc diffusion method and their percentage of inhibition was calculated. Among the methanol and water forms the water form shows more pronounced antibacterial effect against E.coli and less activity in S.aureus.