Bacteria can be easily spread through families and communities due to poor hygiene. Bacteria transmissible through shake hand were experimentally isolated from samples collected from undergraduates students and staff of S.R.R. Govt. Arts & Science College, Karimnagar. The isolated colonies were then sub cultured in nutrient agar slants in order to obtain pure culture of all colonies. All students (100%) found to harbour bacteria on their hands. Bacterial pathogens isolated from the hands of students include Escherichia coli, Streptococcus faecalis, Staphylococcus aureus, Bacillus spp., Klebsiella spp. Pseudomonas spp., Acenetobacter spp. Highest contamination of Bacillus spp was isolated. Showed Percentage of bacterial isolates from the samples collected from hands after calculating the total percentage of each isolate Escherichia coli 30% Streptococcus faecalis 20%, Staphylococcus aureus 26%, Bacillus spp.30%, Klebsiella spp.26% Pseudomonas spp.13%, Acenetobacter spp.13%. The highest prevalence was found in male students than in female students and least prevalence in staff.