Toxic substances present in food, environment and in several drugs can cause DNA damage, chromosomal aberrations and alteration of genetic material leading to cytotoxicity and cancer. Natural compounds can be employed to support innate detoxification systems and protect cellular components. Oxidative stress is a prominent adverse effect of cytotoxic substances. Apocynin, a plant compound, is an inhibitor of NADPH oxidase complex, and thus, a strong antioxidant. In the current study, the cytotoxic/anticytotoxic activity of Apocynin was evaluated in a normal cell line CHOk1 and Cancer cell line HepG2, using the chemotherapy drug cisplatin as the cytotoxic agent. Apocynin did not exhibit cytotoxicity towards either of the cell lines, nor has a synergistic effect with cisplatin. On the other hand, Apocynin shows significant anticytotoxicity as evidenced by the lowering of cisplatin-induced cell inhibition in both the cell lines.