Fishes are excellent sources of easily digestible proteins. Ganjam district of Odisha, India has been bestowed with coast line providing distinctive prospect for marine fishing. The rivers like Rushikulya, Dhanei, Bahuda, Ghodahada are also the sources of fresh water fishes. In the current study, proteins were extracted from muscle and liver tissues of some selected edible fresh water and marine fishes of Ganjam district of Odisha coast. The comparative protein profiles of both muscle and liver tissues were studied. All the samples were repeated five times. The average protein content of 1gm muscle tissues of freshwater fish ranged from 68-110 mg and in liver tissues 68-76 mg and those of marine fish muscle tissues ranged from 44 mg to 94 mg and in liver tissues ranged from 50-82 mg respectively. More protein content is observed in fresh water fish muscle and liver tissues in comparison to marine edible fishes. The variation of concentration may be attributed to feeding behavior and climatic factors which influence the general biochemical composition of fish. Further in silico characterization and homology modeling of these proteins may shed more light on their adaptive radiation correlating nutritive value.