Earthworm’s role as farmer’s friend is well known since time immemorial. In contemporary time, commercial vermin culturists have started promoting a product, called vermiwash. Vermiwash contains enzymes, macro and micronutrients that could promote growth and yield of crops. It can be formed in different ways. In the present study, vermiwash was generated from the earthworm, Eisenia foetida, under in laboratory. This vermiwash was employed in different concentrations of seed germination of (Abelmoschus esculentus) lady’s finger plant. Four combinations were prepared from vermiwash for used in seed germination and biochemical analysis. Unusual concentration of vermiwash like, T1 (0.25%); T2 (0.5%); T3 (0.75%); T4 (1%); control (without vermiwash) as test treatments. Each treatment was made in triplicate to reduce the variations. Experiments stood at 96 hours. The results demonstrate that the maximum shoot length was discovered in T3 (2.1cm) and root length were also recorded in T1 (2.9cm) treated groups. The results were significantly increased (P<0.05). The vermiwash treated groups have 100% seed germination when compared with control. The maximum chlorophyll content was found in T2 (4.91), when opposed to other treatment groups. The highest total protein content was noted in T3 (9.50 mg/g) was observed when objected to control. Hence it can be possible to conclude that the effect of vermiwash on the seed germination and biochemical responses of lady’s finger plants is more significant in comparison to control.