Universally, medicinal plants have been utilized for treatment of a few afflictions. The present investigation was performed to assess against microbial activity and preparatory phytochemical screening of Eclipta alba L. leaf removes. The Eclipta alba L. has a place family Asteraceae. The Eclipta alba were shade dried, controlled and was removed using solvents Methanol and distinctive solvents. The antimicrobial activity test performed by the circle diffusion technique. Preliminary phytochemical examination of the plant removes divisions of Chloroform portion (CFF), Carbon tetrachloride part (CTF) and Aqueous fraction (AQF) showed the closeness of tannin, alkaloaids, flavonoids phenolic social affair and flavonoids. The CTF fraction of E. alba indicated high activity against E. faecalis and E. coli bacteria.