Carotenoids are lipid soluble compounds. Therefore, the amount and type of lipid with which they are consumed may influence their absorption. In this study analyses the effect of seaweed supplemented diet and its skin colour changes in the skin of tiger barb and study the quantitative and qualitative analysis of skin using spectrophotometer and TLC. The present study was undertaken to study the effect of seaweed supplemented diet for different feeding duration (days) fed in tiger barb Puntius tetrazona to assess the enhancement of body colour. The total carotenoid content of the control fish skin sample was 2.917 µg/g tissue and which increased during experimentation from 4.0926 µg/g tissue and 5.1552 µg/g tissues. The trend was noticed in the skin of tiger barb fed with seaweed supplementation diet.