In genetic diversity analysis based on morphology traits and random amplified polymorphic DNA (RAPD) markers was carried out in a set of forty-eight ashwagandha genotypes selected from germplasm maintained at Directorate of Medicinal and Aeromatic Plants Research, Boriavi, Anand (Gujarat) and Department of Plant Breeding and Genetics of MaharanaPratap University of Agriculture and Technology, Udaipur (Rajasthan). Observations were recorded on six different phenotypic traits to study the morphological diversity among the collected germplasm. The genotypes were grouped into seven clusters.Intercluster distance was maximum between clusters II and VII followed by clusters I and II hence they were genetically most diverse. Among the seven characters studied, root dry weight per plant contributed the maximum accounting for 35.52 per cent of total divergence. Further, in RAPD marker analysis, 8 primers were used to study the diversity at molecular level in ashwagandha genotypes. The dendrogram constructed on the basis of these marker data was able to classify all the genotypes of ashwagandha into clusters indicating molecular divergence in the population. Ashwagandha genotypes RAS 32 and RAS 57 had the highest similarity, while genotypes JA 20 and IC 283942 had minimum similarity. Thus genotypes, JA 20 and IC 283942 were most diverse with respect to RAPD markers. The study indicated that the RAPD marker analysis can be a useful tool for the assessment of diversity among ashwagandha genotypes, which will aid its further utilization in crop improvement programs.