In present work silver nanoparticles (AgNps) were synthesized intracellular by using Bacillus megaterium sp. Biomass of Bacillus megaterium were added into the reaction vessel in which 100 ml of silver nitrate (1 mM) solution. The silver nanoparticles were characterized by Transmission Electron Microscopy (TEM), UV-Visible Spectroscopy (UV) And Fourier Transform Infrared Spectroscopy (FTIR). Antibacterial activity was studied against the human pathogenic microorganism such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeureginosa, and Proteus vulgaris.