The objective of the present study is to evaluate and compare the phytochemical constitution of methanolic and petroleum ether extracts and determination of total flavonoid content of three medicinally important herbs Cymbopogon citratus, Ocimum sanctum and Trigonella foenum-graecum. The phytochemical analysis was performed by standard methods to detect the presence of glycosides, saponins, tannins, phenolics, alkaloids, flavonoids, triterpenoids and steroids. The total flavonoid content (TFC) was accessed at 510 nm using Rutin as standard and the calculated amount is found higher in Ocimum sanctum.