The plants are valuable source of new medicinal agents. Medicinal plants have various effects on living system and the contents of medicinal plants are used for the development of new drug compound that are used in various diseases. Dipteracanthus prostatus (Poir.) Nees (Synonym Ruellia prostrata) is a indigenous medicinal plant of family Acanthaceae. The whole pant and parts of plant are medicinally important. To some extent information about its phytochemical is available; very few data is available about its antomy which is very important from pharmacognostic point of view. This paper deals with the micromorphological study of root, stem and leaf of Dipteracanthus prostatus for standardization of drug.