Lignocellulosic biomass is a renewable and abundant resource with great potential for bioconversion to value added bioproducts. Lignin causes the serious pollution of toxins problem in aquatic ecosystem owing to its low biodegradability. In the present study was planned to degradate lingocellulosic material by biological treatment methods such as vermicompost, cow dung, mushroom spent waste. The experiment was designed as T₁ coconut shredder waste only, T₂ substrate + cow dung, T₃ substrate + vermicompost and T₄ substrate + mushroom spent waste. Lignocellulosic wastes was allowed for complete degradation nearly 90 days. During this degradation period, pH, temperature, moisture, total organic carbon, total organic nitrogen, C: N ratio, enzyme assay was estimated. Microbial population was also calculated. Enterobacter sp, Bacillus sp, Klebsiella sp, Pseudomonas sp, Streptomyces sp and Azotobacter sp and Trichoderma sp, Pleurotus sp, Aspergillus sp, Rhizopus sp and P.chrysosporium was identified by using standard manuals. In this result, physical chemical parameters was reduced than initial inoculation. Manganese peroxidase was absent in T₁ and T₄. The lignin peroxidase was present in T₁, T_2,, T₃ and T₄. Although final degradation was observed in 90% level in the T₃ using vermicompost followed by T₂cowdung (75%) The findings has significant potent use in the agriculture for the treatment of lignin degradation and lignin related environment pollutants.