Catunaregam spinosa (Thunb.) Triveng, known locally as Gela, belongs to Rubiaceae family. It is used in Ayurveda to treat various ailments. Secondary metabolites from this plant has been extensively characterized and studied. Catunaregam spinosa (Thunb.) Triveng plants from two different geographical locations, one from a tribal settlement area in North Goa(rural) and another from SGNP, Mumbai(urban), were sampled to understand the effect of geographical locations on the plant’s phytochemical composition. A preliminary phytochemical analysis of total phenolics and flavonoid, along with the antimicrobial activity from leaf and stem samples of both the plants were studied. The leaf sample from Goa showed highest concentration of total phenolics whereas, Mumbai leaf sample showed high flavonoid content and antimicrobial activity with MIC of 18.22mg/ml against Staphylococcus aureus. Thus the two plants from different geographical locations showed comparable difference in some of the phyto constituents and also differed in their antimicrobial activity.