Heavy metal, Lead is a common environmental pollutant released into the surrounding atmosphere as a byproduct of industrial and anthropogenic activities. Lead sources include mining and smelting of ore, manufacture of lead containing products, burning of coal and oil, and waste incineration. Due to the environmental persistence of lead, historic sources also contribute heavily to present contamination. These incorporated leaded gasolines, paints, solder in food cans, pesticides and lead shot and sinkers. Through food chain heavy metal lead can enter fish body subsequently affect human beings. Fish is generally appreciated as one of the healthiest and cheapest source of protein and it has amino acid compositions that are higher in cysteine than most other sources of protein. The aim of the present study was to assess the protein and amino acid levels in gill and kidney of Channa striatus was exposed to sublethal concentration of lead acetate further the fish treated with meso 2,3- dimercapto succinic acid (DMSA) 2.5 ppm and 5 ppm in group 3 and group 4 respectively for the period of 10, 20 and 30 days. The fish exposed to lead acetate showed a decrease the protein and increase the amino acid levels further the fish were treated with dimercaptosuccinic acid showed gradually the protein contents increased and amino acid levels were decreased for the periods of 10, 20 and 30 days in gill and kidney. The objective of the present investigation was to observe the ameliorative performance of dimercaptosuccinic acid reduced the lead acetate induced alterations in protein and amino acid in the gill and kidney of freshwater fish, Channa striatus.