Ticks and tick-borne diseases (TBDs) are major constraint to livestock productivity and fitness in tropical regions. The objectives of the current study were to identify the species composition and to estimate the prevalence and seasonal distribution of tick infestation of dairy cattle in the southern Gezira state, central of Sudan as a preliminary step for more detailed ongoing research in tick-borne diseases in this region. During twelve months, species of ticks infesting dairy cattle in farms from different locations in Gezira State were determined, through winter, summer and autumn. All visible attached ticks (1236) were collected from half body of cattle heads. In each farm, monthly about 100 samples were collected according to animal sex, age (less than one year old, one to three years old and more than three years old) and breed (local breeds, cross [indigenous vs. Friesian, indigenous vs. Jearcy and indigenous vs. Jamoos]). The same animals whenever possible were used during each collection. Tick species belonging to four genera have been identified and the relative prevalence of each species was: Rhipicephalus e. evertsi was the most abundant (46.8%) from the total of the ticks followed by Boophilus decoloratus (14.2%), Amblyomma lepidum (9.20%), Hyalomma anatolicium (7.60%), Hyalomma excavatum (6.33%), Hyalomma detritium (5.97%), Hyalomma impeltatum (2.57%), Boophilus annulatus (2.53%), Rhipicephalus quilhoni (2.53%) and Hyalomma rufipes (1.10%). A significant (P < 0.05) seasonal pattern of ticks’ activities were observed between animals’ breeds, sex and ages. This study ascertained the presence of a broad variety of cattle tick species, with possible implications for dairy cattle production in Gezira State and most of which are of public health importance.