The objective of this study aimed analyzes potential cytotoxicity of the chlorpyrifose and/or glyphosate and protective effect of vitamin C. 110 male albino rats were treated with chlorpyrifose and glyphosate daily for 30 and 60 days. Hepatotoxicity was monitored by quantitative analysis of the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) activities, Gamma-glutamyl transferase GGT, total protein, and albumin. The second aim of this study to investigate effect of used chlorpyrifose or glyphosate alone or together on lipid peroxidation (LPO) levels of animals as an index of antioxidant status and oxidative stress, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and nitric oxide (NO) respectively, Triiodothyronine (T3), thyroxin (T4) and thyroid-stimulating hormone (TSH) and Testosterone (TST) were measured and liver was collected for histopathological study. The results refer to a significant elevation of (MDA and NO) while a significant decrease in other parameters (CAT, GSH and SOD) and increase in liver functions (ASAT, ALAT, GGT and ALP). Also a significant decrease in total protein, albumin level. The hormones were recorded significant increase in T3 and T4 while decrease in TSH and TST in toxicated groups as compared to the control groups. Histopathology revealed vacuolar of hepatocytes with random hepatocyte necrosis and mononuclear cell infiltration. The administration of the vitamin C had beneficial and decrease side effects against the deleterious changes of chlorpyrifose or glyphosate alone or together. In conclusion results suggest a potential contribution of pesticide mixtures to the etiology of some body diseases while vitamin C has beneficial effects as it tends to dampen chlorpyrifose or glyphosate alone or together toxicity in rats.