Ayub Agricultural Research Institute (AARI) is the premier research organization of the country. It has been at the forefront in developing sugarcane production in the country. Sugarcane varieties developed by AARI’s Research System have been widely appreciated and opted by the sugarcane growers of the country. During 2016-17, sugarcane varieties developed by AARI’s research system were planted over 92% sugarcane area of Punjab. The main objectives of the study are to document the spread and adoption of AARI’s sugarcane varieties and assess the impacts of AARI’s sugarcane varieties in Punjab from 2000-01 to 2016-17.The results of the study revealed that sugarcane breeding research has generated enormous benefits during the whole study period. During 2016-17, economic impact of sugarcane breeding research of Ayub Agricultural Research Institute, Faisalabad was estimated at Rupees 38 billion. Economic benefits averaged Rs. 15.85 billion per year during the whole study period.