Documentation of indigenous knowledge through botanical studies is very important for the conservation and utilization of biological resources. The present study aims to document the traditional knowledge of medicinal plants used by the indigenous tribes-Kurichiyas, Kurumas, and Paniyas residing in the pockets of Wayanad district of Kerala. Ethnobotanical information was collected using primary as well as secondary sources of data. The study noticed that the above-mentioned tribes use 114 medicinal flowering plant species belonging to 47 families., among them, 39 are herbs, 28 are shrubs, 26 are trees and remaining 21 are climbers. The difference in usage of plants by same tribes occupying different localities and different tribes of the same or nearby localities was observed. The study also revealed that the Kurichiya community possesses high ethnobotanical knowledge when compared to the other two communities. Ethnomedicinal studies supplemented with global information have great significance in the collection of traditional knowledge, preparation of recorded data and in the conservation of endangered medicinal plant species.