This research experiment was conducted at Sugarcane Research Institute, Faisalabad, Pakistan during crop season 2015-16. The objective of the experiment is to evaluate the ratooning ability of different sugarcane clones at various harvesting dates of cane plant crop. The trial was laid out in Randomized Complete Block Design (split plot arrangement) and replicated thrice. Five sugarcane clones i.e. S2003-US-633, S2006-US-469, S2006-US-658, S2006-US-832 and one check variety HSF-240 were sown in February, 2012 in main plots. The harvesting dates of plant crop viz.15^th November, 15^th December, 15^th January, 15^th February and 15^th March were placed in sub-plots. The highest cane yield was recorded for S2006 US-832 (87.1t ha^-1) as against the lowest in S2006 US-469 (68.5t ha^-1). Among harvesting dates, the cane yield was maximum (91.9 t ha^-1) for 15^th February harvested plant crop and was lowest for ratoon kept on 15^th December (60.1t ha^-1). In interaction of these two factors, clone S2006-US-832 gave more cane yield (100.7 t ha^-1) when plant crop was harvested on 15^th February.