International Journal of Biology Research

International Journal of Biology Research


International Journal of Biology Research
International Journal of Biology Research
Vol. 3, Issue 4 (2018)

Study the effect of different super ovulation hormones (Urinary Human Menopausal Gonadotropin, Urinary Follicular Stimulating hormone or Recombinant Follicular Stimulating Hormone) in combination with Human Chorionic Gonadotropin on mice oocytes yield and quality and embryonic development after in vitro fertilization


Ahmed M Al-Atrash, Ahmed S Abdoon, Samir AM Zaahkouk, Omaima M Kandil, Amal AA Ammar, Asmaa AM Yones

Advanced reproductive techniques enable greater numbers of oocytes or embryos of high quality to be used in basic reproductive research. Therefore, hormonal control for multiple follicular growth in IVF program was directed toward maximizing the yield of fertilizable oocytes. The present study was conducted to evaluate the efficacy of different types of gonadotropin used for induction of superovulation in mouse. 15 IU human menopausal gonadotropin (hMG), Urinary follicle stimulating hormone (U-FSH) and recombinant follicle-stimulating hormone (rFSH) were injected in mouse followed 48 hours later by injection 15 IU human chorionic gonadotropin (hCG). After 14 hours from hCG injection the oocytes were collected and evaluated according to cumulus oocyte complexes (COCs) expansion and oocyte maturation rate. Matured oocytes were In vitro fertilized using mouse epididymal spermatozoa, then IVF oocytes were cultured In vitro to the blastocyst stage. Result indicated that hMG was superior than U-FSH and rFSH. hMG. hMG produced a significantly (P<0.05) higher oocytes yield and quality than (pure FSH) the worst oocyte yield and quality was obtained by using (rFSH). In vitro fertilization, cleavage and blastocyst rates were significantly (P<0.05) higher for hMG group than pure FSH. Nevertheless, in U-FSH group, fertilization and cleavage rates were significantly (P<0.05) higher than oocytes retrieved from rFSH treated animals. In conclusion, 15 IU hMG followed 48h later by injection of 15 IU hCG is the optimal protocol for induction of superovulation in mouse. Also, the developmental competence of oocytes obtained by this protocol was higher than that of U-FSH or rFSH.
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How to cite this article:
Ahmed M Al-Atrash, Ahmed S Abdoon, Samir AM Zaahkouk, Omaima M Kandil, Amal AA Ammar, Asmaa AM Yones. Study the effect of different super ovulation hormones (Urinary Human Menopausal Gonadotropin, Urinary Follicular Stimulating hormone or Recombinant Follicular Stimulating Hormone) in combination with Human Chorionic Gonadotropin on mice oocytes yield and quality and embryonic development after in vitro fertilization. International Journal of Biology Research, Volume 3, Issue 4, 2018, Pages 47-51
International Journal of Biology Research