Polycystic ovary syndrome (PCOS) is a complex endocrine disorder affecting 5-10% of women of reproductive age. The present study is to detect the effect of chromosomal abnormalities and cytogenetic study in cases of polycystic ovary syndrome (PCOS). In the study 100 females were selected from out patients clinic of El Galaa teaching hospital, all cases will be subjected to history taking, radiological examination in addition to hormonal profile and chromosomal study. The obtained results showed some patients with PCOS are a significant change in LH, T test., F test. And PRL were observed. And there no cytogenetic abnormalities has been confirmed in this study.