Indian medicinal herbs and plants are used since ancient times to treat different diseases and ailments as these natural products exert broad-spectrum actions. The present study was aimed to explore the free radical potential of aqueous hot extract of Eugenia uniflora leaves. In vitro assays like DPPH scavenging assay, DMPD scavenging assay, metal chelating ability, FRAP scavenging assay and anti hemolytic assay was carried out using standard procedures. In present investigation, aqueous extract of Eugenia uniflora leaves exhibited high antioxidant properties to scavenge free radicals thus protecting the biomolecules from damage. These results emphasized the benefit of the extract and thus augmented the urge of in vivo studies to further confirm the beneficial effect of these extract.