The endophytic fungi Cheatomium globosum was isolated on potato dextrose agar (PDA) from stems of Dalbergia sisso. The Cheatomium globosum screened for its antimicrobial activity. Antimicrobial activity in ethanolic extract of Cheatomium was tested by using agar well diffusion method. In antibacterial activity Cheatomium globosum showed highest zone of inhibition i.e. 18 mm against Staphylococcus aureus and Bacillus subtillis. Similarly, in antifungal activity the maximum zone of inhibition was 40 mm obtained against Fusarium solani and Aspergillus niger. Cheatomium globosum have potential activity against plant pathogenic fungi and bacteria isolated from Dalbergia sisso.